gyrB [2018-02-06 13:17:56]

DNA gyrase (subunit B)

Locus: BSU00060

Isoelectric point: 5.38

Molecular weight: 71.32 kDa

Protein length: 638 aa

Gene length: 1914 bp

Function: DNA supercoiling, initation of replication cycle and DNA elongation

Product: DNA gyrase (subunit B)

Essential: yes

E.C.: 5.99.1.3

Synonyms: novA

Genomic Context

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Categories containing this gene/protein

• Information processing → Genetics → DNA condensation/ segregation
• Groups of genes → Essential genes
• Groups of genes → Phosphoproteins → Phosphorylation on a Ser residue

Gene

Coordinates: 4,867 → 6,783

Phenotypes of a mutant

• essential PubMed

The protein

Catalyzed reaction/ biological activity

• ATP-dependent breakage, passage and rejoining of double-stranded DNA (according to Swiss-Prot)

Protein family

• type II topoisomerase family (according to Swiss-Prot)

Paralogous protein(s)

• ParE

Domains

• TOPRIM domain (aa 422-536) PubMed

Modification

• phosphorylated on Ser-400 PubMed

Effectors of protein activity

• novobiocin binds to the ATP binding site of GyrB and inhibits ATP hydrolysis and enzymatic activity, this results in replication overinitiation PubMed
• an R138L mutation confers novobiocin resistance PubMed

Structure

• 4I3H (from Streptococcus pneumoniae, 55% identity) PubMed

Localization

• nucleoid-associated in actively growing cells PubMed

Additional information

• GyrB is subject to Clp-dependent proteolysis upon glucose starvation PubMed

Expression and Regulation

Operons

• Genes: yaaA-recF-remB-gyrB-gyrA
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

Labs working on this gene/protein

• Dagmar Klostermeier, University of Münster, Germany homepage

References

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